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Normalize Plates (POC)

Community NodesHCS ToolsNormalization
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Groups of data (e.g. wells of a plates) are normalized relative to a subset (e.g. some wells within that plate, e.g. negative control). For each group the mean (or median) is calculated for the selected subset. Based on this estimate, all data points of each group are normalized by applying this formula:

x.poc = x / mean(x[subset]) * 100
or
x.poc = x / median(x[subset]) * 100

Literature: Malo et al., Nat. Biotechnol. 24, 167-179 (2006)

Node details

Input ports
  1. Type: Table
    Raw data
    Input table
Output ports
  1. Type: Table
    Normalized data
    Table with normalized values
  2. Type: Table
    Statistic
    Table with statistics used for normalization

Extension

The Normalize Plates (POC) node is part of this extension:

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Related workflows & nodes

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