This workflow can be used to fit dose-response curves from normalised biochemical assay data (%Inhibition) using the HCS extension. This workflow needs R-Server to run in the back-end. Start R and run the following command: library(Rserve); Rserve(args = "--vanilla")
IC50 values will not be extrapolated outside the tested concentration range
For activity classification the following criteria are applied:
- maximum (average % inhibion) >25 % and slope is >0 and IC50 > 5 µM or
- minimum (average % inhibion) >75 %
Results are formatted for upload to the European Chemical Biology Database (ECBD)
Workflow
DRC_biochemical_toECBD
Used extensions & nodes
Created with KNIME Analytics Platform version 4.5.2
- Go to item
KNIME HCS Tools
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany
Version 4.0.0
- Go to item
- Go to item
- Go to item
- Go to item
- Go to item
- Go to item
Legal
By using or downloading the workflow, you agree to our terms and conditions.