This workflow measures channel shift (caused by chromatic aberration and/or camera misalignment) from 3-channel images of fluorescent beads, and applies the measured transformation to correct track coordinates.
It requires the ‘FMI KNIME Plugins’ extension from the following update site:
https://community.knime.org/download/ch.fmi.knime.plugins.update/
Workflow
Channel Shift (3 Channels) Measurement and Track Correction
Used extensions & nodes
Created with KNIME Analytics Platform version 4.0.2 Note: Not all extensions may be displayed.
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KNIME AG, Zurich, Switzerland
Version 4.0.2
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KNIME AG, Zurich, Switzerland
Version 4.0.0
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KNIME AG, Zurich, Switzerland
Version 4.0.1
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University of Konstanz / KNIME
Version 1.8.0
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KNIME / LOCI
Version 0.11.6
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KNIME AG, Zurich, Switzerland
Version 4.0.1
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KNIME AG, Zurich, Switzerland
Version 4.0.2
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KNIME AG, Zurich, Switzerland
Version 4.0.2
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KNIME AG, Zurich, Switzerland
Version 4.0.0
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KNIME AG, Zurich, Switzerland
Version 4.0.2
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KNIME AG, Zurich, Switzerland
Version 4.0.0
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